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Invitrogen™ PARP1 (cleaved Asp214, Asp215) Polyclonal Antibody
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Product Code. 10566463
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Product Code. 10566463 Supplier Invitrogen™ Supplier No. 44698G

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Description

Rabbit Polyclonal Antibody

This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. The antibody has been used in western blotting and previous lots have been used in immunostaining applications. Positive controls used in western blotting were Jurkat or HeLa cells treated with staurosporine or etoposide (25 μM for 3 hours).

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
TRUSTED_SUSTAINABILITY

Specifications

Antigen PARP1 (cleaved Asp214, Asp215)
Applications Immunohistochemistry (Paraffin), Western Blot
Classification Polyclonal
Conjugate Unconjugated
Formulation Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
Gene PARP1
Gene Accession No. P09874, P11103, P18493, P27008
Gene Alias 5830444G22Rik; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); Adprp; Adprt; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; Parp1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1; sPARP-1; synthetase fragment (AA 67); Unknown (protein for IMAGE:8023736); unnamed protein product
Gene Symbols PARP1
Host Species Rabbit
Immunogen The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 11545, 142, 25591, 286764
Target Species Human, Mouse, Rat, Bovine
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG
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