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Invitrogen™ Human Trappin-2/PI3 ELISA Kit

Produktkode 17195539
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Quantity:
5 x 96 Tests
96 Tests
Förpackningsstorlek
1 set
Each
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Produktkode 17195539

missing translation for 'mfr': Invitrogen™ EHPI3X5

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ELISA

The Human Trappin-2/PI3 ELISA quantitates Hu PI3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PI3. Principle of the method The Human PI3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
TRUSTED_SUSTAINABILITY

Tekniske data

Accession Number P19957
Assay Range 6.86 to 5000 pg/mL
Assay Sensitivity 5 pg/mL
Calibration Range 5 pg/mL to 5000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Proteases, Peptidases & Inhibitors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Sufficient For 5 x 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 5266
Gene Symbol PI3
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Research Discipline Other Research
Sample Volume Plasma 3.3 μL, Serum 3.3 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym ESI, SKALP, WAP3, WFDC14, cementoin
Target PI3
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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