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TaqMan® TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

101.00 EUR - 1160.00 EUR

Specifications

Detection Method Primer-probe
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Fast
Green Features Sustainable packaging
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Products 6
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10794597
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TaqMan®
4331182
S (250 x 250 μL Reactions), inventoried
259.00 EUR
Each
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11997011
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TaqMan®
4351368
L (2900 x 967 μL Reactions), made to order
1160.00 EUR
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11620099
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TaqMan®
4351370
M (750 x 750 μL Reactions), made to order
560.00 EUR
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10330545
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TaqMan®
4351372
S (360 x 360 μL Reactions), made to order
429.00 EUR
360µL
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11967021
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TaqMan®
4448892
XS (75 x 75 μL Reactions), made to order
101.00 EUR
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Estimated Shipment: 15-04-2024
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TaqMan®
4453320
XS (75 x 75 μL Reactions), inventoried
102.00 EUR
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Description

Description

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

Features of TaqMan Gene Expression assays include:
Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
Accurate—identify small fold-changes with high accuracy of quantitation
Extensive content—Over 1.8 million predesigned assays available for over 25 different species
Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
Fast delivery—your assays shipped in as little as 1–2 days (see below)

Approximate ship time
Inventoried: 1–2 days in North America, 3–5 days in Europe*
Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.**

Recommended master mix: TaqMan Fast Advanced Master Mix (Cat. No. 4444556)

*Our most popular assays are stocked in North America and Europe for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.

**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

Specifications

Specifications

Primer-probe
qPCR
Sustainable packaging
Gene Expression Assay
60 X
High
Fast
TaqMan™
Room Temperature
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.