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Applied Biosystems™ TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited
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Product Code. 15601812 Shop All Applied Biosystems Products
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Quantity:
250 reactions
Unit Size:
250 reactions
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Product Code. 15601812

Brand: Applied Biosystems™ 4485713

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205.40 EUR valid until 2025-12-19
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This item is not returnable. View return policy

Description

Provides a pre-formulated assay for quantitating human GAPDH.

This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of a JUN™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers. The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress GAPDH.

Save time in assay design, formulation, and testing by using the TaqMan™ GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high GAPDH expressors.

The JUN™ dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN™ dye) due to an overlap of spectra. TaqMan™ Multiplex Master Mix with MUSTANG PURPLE™ dye is recommended.

  • Eliminate assay design time by using pre-designed primers and pre-designed JUN™ dye-labeled TaqMan™ probe
  • Minimize experimental optimization time
  • Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays
  • Formulated at a primer-limited concentration for use in multiplex reactions
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_002046.3
Amplicon Size 118
Content And Storage Primer-Probe Set(s)
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Gene Symbol GAPDH
Green Features Sustainable packaging
Internal Probe Modification QSY™ Non-Fluorescent Quencher (3')
Label or Dye JUN
Product Line TaqMan
Product Type Gapdh Assay
Quantity 250 reactions
Species Human
Sufficient For 250 Reactions
Target Glyceraldehyde-3-phosphate dehydrogenase
For Use With (Application) Gene Expression
No. of Reactions 250 Reactions
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For Research Use Only. Not for use in diagnostic procedures.

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