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Thermo Scientific™ PCR Master Mix (2X)

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.

Brand:  Thermo Scientific™ K0172

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Product Code. 11863913

  • 495.00 EUR / 1000 reactions

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Description

Description

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Convenient, ready-to-use mix
  • Thermostable—half life is more than 40 min. at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • High throughput PCR
  • DNA labeling

Note

The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 2X PCR Master Mix, 20 x 1.25 mL
• Nuclease-free water, 20 x 1.25 mL

Store at -20°C.
Taq DNA Polymerase
PCR Master Mix
Standard PCR
1X
1000 Reactions
SuperMix or Master Mix
Low
Standard
Dry Ice
2X
No
3'-A
DNA
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For Research Use Only. Not for use in diagnostic procedures.