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IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression
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Beschreibung
pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.
- NeoR for generation of stable cells lines.
- Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- ColE1 + Amp: for propagation in E. coli
- NeoR: neomycin resistance gene to generate stable cell lines
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Spezifikation
Spezifikation
| Format | Liquid |
| For Use With (Equipment) | StarGate™ System, Mammalian Host Cells |
| Inducing Agent | SV40 large T antigen (e.g. COS1 or COS7) |
| Includes | Neomycin resistance gene |
| pH | 8 |
| Promoter | Human CMV Immediate-early |
| Product Type | Acceptor Vector for mammalian expression |
| Storage Buffer | 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg |
| Content And Storage | 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Protein Tag | Strep-tag for N-terminal and FLAG-tag for C-terminal |
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