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R&D Systems™ Recombinant Human u-Plasminogen Activator/Urokinase Avi, CF
Description
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/μg, as measured under the described conditions. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human u-Plasminogen Activator (uPA)/Urokinase His-tag Avi-tag (Catalog # AVI1310) binds to Human u-Plasminogen Activator (uPA) Antibody (Catalog # MAB1310 ) with an ED50 of 0.300-4.50 ng/mL.
Specifications
Specifications
| Accession Number | P00749.2 |
| For Use With (Application) | Enzyme Activity |
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl and CaCl2. |
| Gene ID (Entrez) | 5328 |
| Molecular Weight (g/mol) | MolecularWeight-observed: 19-20 kDa & 32-38 kDa, under reducing conditions., MolecularWeight-theroretical: 18 kDa (long A chain), 3 kDa (short A chain), 31 kDa (B chain) |
| Quantity | 20 μg |
| Source | Human embryonic kidney cell, HEK293-derived human u-Plasminogen Activator (uPA)/Urokinase protein Met1-Leu431 with C-terminal 6-His and Avi-tag |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening. |
| Endotoxin Concentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Gene Alias | ATF, EC 3.4.21, EC 3.4.21.73, plasminogen activator, urokinase, PLAU, uPA, u-PA, uPlasminogen Activator, U-plasminogen activator, urinary, Urokinase, urokinase-type plasminogen activator |
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