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R&D Systems™ Recombinant Human TNF-alpha (HEK293-expressed) Protein

Product Code. 18725646
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Quantity:
1 mg
100 μg
20 μg
250 μg
50 μg
500 μg
Unit Size:
100µg
1mg
20µg
250µg
500µg
50µg
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Product Code. 18725646

Brand: R&D Systems™ 10291TA020

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Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. ED50 for this effect is 20 to 100 pg/mL.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01375
For Use With (Application) Bioactivity
Gene ID (Entrez) 7124
Molecular Weight (g/mol) 17 kDa
Name APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Quantity 20 μg
Source Human embryonic kidney cell, HEK293-derived human TNF-alpha protein Val77-Leu233
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Conjugate Unconjugated
Species Human
Reconstitution Reconstitute at 500 μg/mL in PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
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