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R&D Systems™ Recombinant Human PDGF-BB, Animal-Free Protein

Product Code. p-7182946
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Quantity:
10 μg
50 μg
Unit Size:
10µg
50µg
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Product Code. 18785396

Brand: R&D Systems™ AFL220010

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This item is not returnable. View return policy

Description

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells

  • The ED50 for this effect is 1.5 to 6 ng/mL.
  • The specific activity of Recombinant Human PDGF-BB is approximately 5.8 x 103 IU/μg, which is calibrated against Recombinant Human PDGF-BB WHO International Standard a(NIBSC code: 94/728).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01127
For Use With (Application) Bioactivity
Gene ID (Entrez) 5155
Molecular Weight (g/mol) 12.3 kDa (monomer)
Quantity 10 μg
Source E. coli-derived human PDGF-BB protein Ser82-Thr190 Produced using non-animal reagents in an animal-free laboratory.
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Gene Symbol PDGFB
Conjugate Unconjugated
Species Human
Reconstitution Reconstitute at 0.2 mg/mL in sterile 4 mM HCl.
Buffer Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Endotoxin Level <0.01 EU per 1 μg of the protein by the LAL method.
Purity or Quality Grade >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
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