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  5. Invitrogen™ Feline RANTES ELISA Kit

Invitrogen™ Feline RANTES ELISA Kit

Product Code. p-7230674
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
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Product Code. 15454883

Brand: Invitrogen™ EFCCL5

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Description

ELISA

The Feline RANTES/CCL5 ELISA quantitates Cat RANTES in feline serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Cat RANTES. Principle of the method The Feline RANTES solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q8SQ40
Assay Range 0.82 to 200 pg/mL
Assay Sensitivity 0.7 pg/mL
Calibration Range 0.7 pg/mL to 200 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family GCPR
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA antibody pair detects Feline Rantes. Other species not determined.
Sufficient For 96 Assays
Target Species Feline
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 493689
Gene Symbol CCL5
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Inflammation
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym RANTES
Target CCL5 (RANTES)
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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