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Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Brand: Thermo Scientific™ A35349
Additional Details : Weight : 0.00100kg Transport : UN number : 3261 Chem class : 8 Pack group : II
Description
Thermo Scientific™ Pierce TCEP-HCl is pure, crystalline Tris(2-carboxyethyl)phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable.
Thermo Scientific No-Weigh products are specialty reagents provided in pre-aliquoted format. The pre-weighed packaging helps prevent loss of reagent reactivity and contamination over time by minimizing repetitive opening and closing of vial. The format enables use of fresh vial of reagent each time, eliminating hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
- Odorless: Unlike DTT or BME, TCEP is odor-free and can reduce proteins conveniently on bench
- Specific: Selective and complete reduction of even most stable water-soluble disulfides over wide pH range
- Simple: Effective reduction at room temperature and pH 5 in less than five minutes
- Stable: Inherent stability and resistant to air oxidation eliminates need for any special precautions while handling or storing; non-volatile and non-reactive toward other functional groups found in proteins
- Efficient: For most applications, 5 to 50mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within few minutes at room temperature
- Compatible: With TCEP, removal of reducing agent is not necessary prior to most applications, (e.g. histidine-tagged protein purification, maleimide conjugations)
Specifications
| Store at room temperature in a sealed container to prevent oxidation. | |
| No-Weigh™, Pierce™ | |
| 10 x 1 mg |
| Room Temperature | |
| Solid |
For Research Use Only. Not for use in diagnostic procedures.