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Invitrogen™ PARP (Cleaved) Multispecies In-Cell ELISA Kit, Colorimetric

Product Code. 11558100
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96 Tests
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11558100 96 Tests Each
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Product Code. 11558100 Supplier Invitrogen™ Supplier No. 62219

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Description

In-cell ELISA Kit

The Cleaved PARP Multispecies In-Cell ELISA Colorimetric Detection Kit is a simple method for quantifying intracellular proteins in whole cells. Principle of the method To perform the assay, cells are first plated, treated and fixed. Expression of the protein(s) of interest is monitored in wells of a microplate using target-specific primary antibodies (see the Important Product Information section for antibodies included in each kit) and a horseradish peroxidase (HRP)-conjugated detection reagent. The kit is supplied with a whole-cell stain to control for differences in cell plating, which is important when measuring relative levels of a protein with different treatments or assessing its post-translational modification (PTM) form. After staining, the results are analyzed by normalizing the absorbance (HRP activity) values to cell number, which adjusts for the cell plating differences among the wells. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P09874
Description Pierce Cleaved PARP Colorimetric In-Cell ELISA Kit
Conjugate HRP
Immunoassay Kit Format Multi-component kit with HRP-conjugate
Product Type ELISA Kit
Sample Type Supernatant
Sufficient For 96 microplate wells of overnight-cultured cells
Target Species Human
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 142
Gene Symbol ADPRT,ADPRT 1,ADPRT1,ARTD1,PARP,PARP-1,PPOL,pADPRT-1
Kit Contents Blocking Buffer, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, HRP Conjugate, TMB Substrate, TMB Stop Solution, Janus Green Whole-Cell Stain, Elution Buffer, Thin Plate Seal Assembly, Components included only in the target-specific kits:Antibody #1 and Antibody #2, Anti-Cleaved PARP Antibody
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Supernatant, 10,000 cells
Storage Requirements 2°C to 8°C
Target Human
Test Time 1 hr. 20 min.
Total Assay Time 24 hr.
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