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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up
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No. of Reactions:
240 Preps
50 Preps
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240 preps
50 reactions
Beschreibung
NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.
- Two applications in one kit—One buffer to process both sample types, with only one loading step
- High recovery for fragments down to 50 bp—Average recovery from 60–90%
- Minimized elution volume of 15μL—Highly concentrated DNA
- Binding buffer with pH indicator—Ensures optimal binding conditions
- Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
- Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
- Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
- Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)
Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer
Spezifikation
Spezifikation
| Color | pH Indicator: Yellow = correct conditions Green = pH slightly high (adjustment recommended) Blue = pH too high (adjustment necessary) |
| Format | Mini Spin Column |
| Isolation Technology | Silica Membrane Technology |
| Efficiency | Typical recovery: 70–95% |
| Elution Volume | Elution volume: 15–30μL |
| For Use With (Equipment) | TAE or TBE agarose gels, PCR |
| Includes | Binding Buffer NTI (40mL), Wash Buffer NT3 Concentrate (25mL), Elution Buffer NE (13mL), NucleoSpin Gel and PCR Clean-up Columns (50), 2mL Collection Tubes (50) |
| No. of Reactions | 50 Preps |
| Quantity | 1 Kit |
| Purity | A260/A280: 1.8–1.9 |
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Sicherheit und Handhabung
Signalwort
- Warning
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
- MIXTURE LIST-Contains : guanidinium thiocyanate
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