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  5. Macherey-Nagel™ NucleoSpin™ Blood XL, Maxi kit for DNA from blood

Macherey-Nagel™ NucleoSpin™ Blood XL, Maxi kit for DNA from blood

Product Code. 12731021
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12731021 10 10 reactions
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Product Code. 12731021 Supplier Macherey-Nagel™ Supplier No. 740950.10

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Description

Large scale purification of high quality DNA from blood.

NucleoSpin™ Blood XL is designed for the rapid, large-scale preparation of highly pure genomic DNA from up to 10 mL whole blood, serum, plasma or other body fluids. Buffy coat volumes up to 2mL can be processed easily. The binding capacity of the NucleoSpin™ Blood XL columns is about 700μg of genomic DNA. The kit allows preparation of pure genomic DNA from 2mL to 10 mL of human whole blood with an A260/280 ratio between 1.7 and 1.9.Typical DNA yields – depending on the concentration of leukocytes in the respective blood sample – are in the range of 20μg to 30μg per mL of healthy human blood. Blood treated either with EDTA, citrate, heparin or CPDA can be used. Time for preparation of genomic DNA from blood with NucleoSpin™ Blood XL is approximately 1 hour. The obtained DNA can be used directly as template for PCR* reactions or can be subjected to endonuclease digestion. Kit components: NucleoSpin™ Blood XL columns, 50 mL collecting tubes, 50 mL elution tubes, buffers, proteinase K.
  • Silica membrane technology
  • Yield: 200μg to 300μg
  • Sample material: ≤10 mL/108 cells
  • Elution volume: 500μL to 2, 000μL
  • Binding capacity: >1,000μg
  • Preparation time: 1hr/prep
  • Format: mini spin column
  • No use of organic solvents For rapid isolation of genomic DNA from whole blood, serum, plasma or other body fluids.

Specifications

Detection Method Silica-membrane Technology
Format Maxi Spin Column
For Use With (Application) Whole Blood, Treated with Citrate, EDTA, Heparin, CPDA
Includes 2mL Collection Tubes, Buffers, Proteinase K
No. of Reactions 10
Product Type NucleoSpin™ Blood XL Column
Quantity 1 Each
Test Time 60min.
Yield 200 to 300μg

Safety and Handling

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