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  6. Macherey-Nagel™ NucleoBond™ PC EF Kit Buffer Solutions

Macherey-Nagel™ NucleoBond™ PC EF Kit Buffer Solutions

Product Code. 15829117
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Quantity:
600 mL
1000 mL
Chemical Name or Material:
Acetate buffer solution
Elution Buffer N5-EF
Equilibration Buffer N2-EF
Lysis Buffer S2-EF
Resuspension Buffer S1-EF
Wash Buffer N3-EF
Wash Buffer N4-EF
Water
Unit Size:
1 L
600mL
8 product options available for selection
Product selection table with 8 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity Chemical Name or Material unitSize
15829117 600 mL Elution Buffer N5-EF 600mL
15869107 1000 mL Resuspension Buffer S1-EF 1 L
15879107 1000 mL Lysis Buffer S2-EF 1 L
15889107 1000 mL Acetate buffer solution 1 L
15899107 1000 mL Equilibration Buffer N2-EF 1 L
15809117 1000 mL Wash Buffer N3-EF 1 L
15819117 1000 mL Wash Buffer N4-EF 1 L
15849117 1000 mL Water 1 L
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Product Code. 15829117 Supplier Macherey-Nagel™ Supplier No. 740796.1

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Description

Additional supply of buffer for use with NucleoBond™ PC EF kits (PC 500 EF, PC 2000 EF, PC 10000 EF, PC Prep 100) for the purification of endotoxin-free plasmid DNA. Buffer solutions include: S1/S2/S3-EF, N2/N3/N4/N5-EF, TE-EF, and H2O-EF.

NucleBond™ PC EF: The classic anion exchanger for purification of endotoxin-free plasmid DNA

  • Proven anion-exchange technology – transfection-grade plasmid DNA
  • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells
  • Patented endotoxin removal by additional washing step – no extra ice incubation necessary
  • Fast and easy clarification of lysates by Folded Filters – no centrifugation required, no shearing forces
  • NucleoBond™ Finalizers available to avoid time consuming centrifugation for plasmid precipitation
  • Separate binding columns of NucleoBond™ PC kits available as NucleoBond™ AX columns

Specifications

Chemical Name or Material Elution Buffer N5-EF
CAS 64-17-5d
Color Colorless
Concentration Ethanol, diluted < 20%; Potassium chloride; Water
For Use With (Application) Purification of endotoxin-free plasmid DNA
For Use With (Equipment) NucleoBond™ PC EF kits (PC 500 EF, PC 2000 EF, PC 10000 EF, PC Prep 100)
pH 6.7 to 7.2
Physical Form Liquid
Quantity 600 mL
Formulation Ethanol, diluted < 20%; Potassium chloride; Water
Odor Alcoholic
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Safety and Handling

compliance-icons
Product Identifier
  • Buffer N5-EF (600 mL)
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
 Supplemental information
  • MIXTURE LIST-Contain: ethanol
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