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Invitrogen™ Human IL-6 ELISA Kit

Product Code. 10503405
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Quantity:
96 Tests
Unit Size:
96 tests
This item is not returnable. View return policy

Product Code. 10503405

Brand: Invitrogen™ KAC1261

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554.80 EUR valid until 2025-12-31
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This item is not returnable. View return policy

Description

ELISA

The Human Interleukin 6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05231
Assay Range 16 to 1690 pg/mL
Assay Sensitivity 2 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) Colorimetric
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3569
Gene Symbol BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
Interassay CV 4.9%
Intraassay CV 5.4
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
Label or Dye HRP
Precision 2 pg/mL
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target IL-6
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 15 mins
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Safety and Handling

compliance-icons
Product Identifier
  • Human IL-6 ELISA Kit
 Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Skin corrosion/irritation Category 1 B
 Hazard Statement
  • H314-Causes severe skin burns and eye damage.
  • H318-Causes serious eye damage.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P363-Wash contaminated clothing before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Hydrogen chloride
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