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BD Biosciences™ NKTEST™ (CE/IVD)

Product Code. 15991230
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Description:
100 Tests
Unit Size:
Each
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Product Code. 15991230

Brand: BD Biosciences™ 659179

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Description

For quantitative determination of the cytotoxic activity of human natural killer (NK) cells.

  • This reagent kit allows the quantitative determination of the cytotoxic activity of human natural killer (NK) cells.
  • Contains cryopreserved prestained K562 target cells, complete medium and a DNA-staining reagent.
  • The K562 target cells are labelled with a lipohilic green fluorescent membrane dye in order to discriminate effector and target cells.
  • After the incubation period in the cytotoxicity assay, killed target cells are identified by a DNA-stain, which penetrates the dead cells and specifically stains their nuclei.
  • In this manner, the percentage of target cells killed by effector NK cells can be determined.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Reagent A (complete medium) and reagent C (DNA staining solution) must be stored at 2-8°C (in refrigerator). Store Reagent B (Interleukin-2) at >-15°C. The K562 target cells have to be stored at - 70°C
Description 100 Tests
Detection Method Flow Cytometry, Stain
Quantity 5 x 100 mL bottles Complete Medium, 5 x 300 μL vials Interleukin-2 (200 U/mL), 5 mL DNA Staining Solution, 5 vials of cryopreserved, prestained K562 Target Cells
Stability Cells are stable for 12 weeks showing a viability >90%.
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