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Invitrogen™ Nitric Oxide Assay Kit

Product Code. 10752345
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Quantity:
2 x 96 tests
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10752345

Brand: Invitrogen™ EMSNO

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This item is not returnable. View return policy

Description

Measure nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in serum, plasma, urine, saliva and other biological fluids.

Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.

Additional Information and Online Ordering

Nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. The interaction of nitric oxide (NO) in a system is measured by the determination of both nitrate and nitrite concentrations in the sample. The relative levels of nitrite and nitrate can vary substantially, therefore the most accurate determination of total NO production requires measurement of both nitrate and nitrite.

  • Target: Nitric oxide (NO) and relative levels of nitrite (NO2-) and nitrate (NO3-)

  • Format: Colorimetric stain (azo dye) for clear 96-well plates

  • Measurement: Absorbance at 540nm (± 20nm)

  • Assay Range: 0 to 100μM

  • Sensitivity: 0.222μM nitrite; 0.625μM nitrate

  • Precision: Dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)

  • Sample Types: Urine, saliva, plasma, serum and other biological fluids

  • Total Assay Time: 15 minutes (nitrite assay); 45 minutes (nitrate assay)

  • Sample Size: 50μL

Includes:

Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.

TRUSTED_SUSTAINABILITY

Specifications

Quantity 2 x 96 tests

Safety and Handling

compliance-icons
Product Identifier
  • Nitric Oxide Assay Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Supplemental information
  • MIXTURE LIST-Contains : hydrogen chloride

For Research Use Only. Not for use in diagnostic procedures.

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