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Invitrogen™ IL-4 Mouse ELISA Kit, High Sensitivity

Product Code. 15542037
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Quantity:
96 Tests
Unit Size:
96 tests
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Product Code. 15542037

Brand: Invitrogen™ BMS613HS

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Description

Includes

  • Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-4
  • Biotin-Conjugate anti-mouse IL-4 monoclonal antibody
  • Streptavidin-HRP
  • Mouse IL-4 Standard lyophilized, 500pg/mL upon reconstitution
  • Sample Diluent
  • Assay Diluent
  • Conjugate Diluent Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  • Amplification Diluent Concentrate (2x)
  • Amplification Reagent I
  • Amplification Reagent II
  • Substrate Solution
  • Stop Solution (1M Phosphoric acid)
  • Adhesive Films

High Sensitivity Sandwich ELISA Kit

The Mouse High Sensitivity Interleukin 4 (HS IL-4) ELISA quantitates Ms HS IL-4 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms HS IL-4. Principle of the method The Mouse HS IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P07750
Assay Range 0.63-40 pg/mL
Assay Sensitivity 0.32 pg/mL
Conjugate Biotin
Product Type ELISA Kit
Sample Type Plasma, Serum, Supernatant
Target Species Mouse
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 16189
Gene Symbol BSF-1,IL-4
Interassay CV 7.9%
Intraassay CV 7.1%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Amplification Diluent Concentrate, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Storage Requirements 2°C to 8°C
Synonym B-cell stimulatory factor 1, B cell growth factor 1
Target Mouse
Test Time 1 hr. 20 min.
Total Assay Time 18 hr.
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For Research Use Only

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