Invitrogen™ Glutathione Ethyl Ester, Biotin Amide (BioGEE) (Glutathiolation Detection Reagent) - Special Packaging

Biotinylated glutathione ethyl ester (BioGEE) is cell-permeant, biotinylated glutathione analog for detection of glutathiolation Under conditions of oxidative stress, cells may transiently incorporate glutathione into proteins. Stressed cells incubated in BioGEE will also incorporate this biotinylated glutathione derivative into proteins, facilitating the identification of oxidation-sensitive proteins.

• Glutathiolation levels can be detecte with fluorescent streptavidin conjugate using either flow cytometry or fluorescence microscopy
• Proteins glutathiolated with BioGEE can also be extracted and analyzed by mass spectrometry or by Western blotting methods in conjunction with fluorophore- or enzyme-labeled streptavidin conjugates

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Glutathione Detection, Nitro-Oxidative Stress, Reactive Oxygen Species