Invitrogen™ CellLight™ Null (control), BacMam 2.0

CellLight Null Control contains no mammalian promoter and no transgene. CellLight reagents combine the utility and selectivity of fluorescent proteins with the transduction efficiency of BacMam technology, enabling unambiguous staining of organelles, cellular structures, and processes in live mammalian cells. They are provided in a ready-to-use format—simply add, incubate, and image—with highly efficient expression in cell lines, primary cells, stem cells, and neurons.Other CellLight reagents are fusion constructs of signal peptides or cell structure proteins with premiere emGFP, TagRFP or CFP for accurate and specific targeting to sub-cellular compartments and structures. A variety of targets, including cytoskeleton, mitochondria, and secretory compartments, are available for convenient multiplexing, co-localization studies, and imaging of dynamic cellular processes where high spatial and temporal resolution is required. The CellLight reagents tolerate fixation with formaldehyde and are therefore compatible with fixed-cell analysis.

• Highly Efficient: >90% transduction of wide range of mammalian cell lines, including primary cells, stem cells, and neurons
• Fast and Convenient: Simply add CellLight Null Control reagent to cells, incubate overnight, and evaluate how well cells tolerate BacMam transduction
• Safe: CellLight reagents are non-replicating in mammalian cells, lack of observable cytopathic effect, and are suitable for biosafety level (BSL) 1 handling

Cell Analysis, Cell Viability & Cytotoxicity, Cell Viability, Proliferation & Function