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Invitrogen™ Human MIF ELISA Kit

Product Code. 17777973
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10 x 96 Tests
Unit Size:
Pack of 10
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17777973 10 x 96 Tests Pack of 10
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Product Code. 17777973 Supplier Invitrogen™ Supplier No. EHMIFX10

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Description

ELISA

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P14174
Assay Range 8.23 to 6000 pg/mL
Assay Sensitivity 6 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4282
Gene Symbol Mif
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target MIF
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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