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Description
Applied Biosystems™ Megaplex™ Primer Pools, Rodent Pool A is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools A. Megaplex™ RT Primers, Rodent Pools A contains RT primers for 335 and 226 unique microRNAs for mouse and rat respectively plus 4 species specific controls. For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Rodent Pool B. Rodent Pool A is intended for use with the TaqMan™ Rodent MicroRNA A Array v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan™ MicroRNA Assays.
Specifications
Specifications
| Concentration | 10X |
| Content And Storage | Contains 150μL of 10X primers (Megaplex™ PreAmp Primers, Rodent Pool A) and 50μL of 10X primer (Megaplex™ RT Primers, Rodent Pool A). Store at -15 to -25°C. |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Primer | RT Primers, PreAmp Primers |
| Product Line | Megaplex, TaqMan |
| Product Type | Primer Pool |
| Quantity | 50 reactions |
| Species | Rat, Mouse |
| Sufficient For | 50 Reactions |
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For Research Use Only. Not for use in diagnostic procedures.
Product Title
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