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  6. Invitrogen™ LysoLight™ Antibody Labeling Kits

Invitrogen™ LysoLight™ Antibody Labeling Kits

Product Code. 18051853
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Quantity:
3 x 1 mg
3 x 100 μg
500 μg
Product Type:
Amine Reactive Dye
Antibody Labeling Kit
Color:
Deep Red
Green
Unit Size:
0.5mg
1mg
300µg
Each
Pack of 3
8 product options available for selection
Product selection table with 8 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity Product Type Color unitSize
18051853 3 x 100 μg Antibody Labeling Kit Deep Red Each
18041853 3 x 1 mg Antibody Labeling Kit Deep Red Each
18014120 3 x 100 μg Amine Reactive Dye Deep Red 300µg
18034070 500 μg Amine Reactive Dye Deep Red 0.5mg
30245367 3 x 100 μg Antibody Labeling Kit Green Each
30245299 3 x 1 mg Antibody Labeling Kit Green Each
30245199 3 x 100 μg Amine Reactive Dye Green Pack of 3
30245107 500 μg Amine Reactive Dye Green 1mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 8 options available.
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This item is not returnable. View return policy
Product Code. 18051853 Supplier Invitrogen™ Supplier No. L36001

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570.00 EUR valid until 2026-03-27
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This item is not returnable. View return policy

Description

The LysoLight Antibody Labeling Kits offer an efficient method for conjugating antibodies with LysoLight dyes. This process allows for specific and sensitive detection of internalized antibodies within lysosomes using fluorescence microscopy, flow cytometry, or high content imaging platforms.

LysoLight Antibody Labeling Kits provide a powerful tool for visualizing the lysosomal localization and catabolic degradation of antibodies and proteins. These kits utilize a unique fluorophore that is only active upon enzymatic cleavage by the lysosomal specific enzyme, Cathepsin B. Outside of the lysosome, LysoLight dyes have little to no observable background fluorescence, and only upon localization to the protease rich environment of the lysosome, does LysoLight become activated and brightly fluoresce bright green or deep red in color.

The labeling protocol provided in the LysoLight Antibody Labeling Kits involves a simple and rapid procedure, ensuring minimal hands-on time and reliable results. The kits include all necessary reagents, including the LysoLight dye, buffers, and purification columns, to facilitate the antibody labeling process. The LysoLight reactive dye contained in these kits readily reacts with lysine residues in antibodies or proteins to yield a covalently attached fluorogenic sensor. The spin columns included in the kits are used for purifying the labeled antibody from excess dye with yields of 70–95%. The kits contain sufficient reagents for three labeling reactions of 100 μg or 1 mg of antibody. The user guide describes a general protocol for using the amine-reactive form of the LysoLight dye for labeling either 100 μg or 1 mg of IgG.
TRUSTED_SUSTAINABILITY

Specifications

Color Deep Red
Description LysoLight™ Deep Red Antibody Labeling Kit
Detection Method Fluorescence
For Use With (Application) Antibody and Protein Labeling
For Use With (Equipment) Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product Type Antibody Labeling Kit
Product Line LysoLight
Quantity 3 x 100 μg
Solubility DMSO (Dimethylsulfoxide)
Excitation/Emission 650/668 nm

For Research Use Only.

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