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Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit
Description
Includes
The PureLink HiPure Plasmid FP Maxiprep Kit includes Equilibration Buffer, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, RNase A, Wash Buffer, Elution Buffer, TE Buffer, HiPure Filter Columns, and a PureLink HiPure Precipitator Module. The PureLink HiPure Precipitator Module includes HiPure Precipitators, and 5mL and 30mL syringes.
Transfection grade. The PureLink HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA. Using HiPure Filter Columns and HiPure Precipitators supplied in kit, you can purify plasmid DNA without centrifugation steps required in traditional isolation protocols. The quality of purified plasmid DNA is equivalent to that obtained by two passes through cesium chloride gradients - most rigorous method for DNA purification - making it suitable for transfection and other applications requiring high-purity plasmid DNA.
- Simple protocols—bacterial lysate clarification and isopropanol precipitation without centrifugation
- Fast results—from E. coli cell pellet to high-purity, transfection-grade plasmid DNA in about 60 minutes
- High yields—up to 850μg from a high-copy plasmid
Specifications
Specifications
| Column Type | Filter Column |
| Content And Storage | The PureLink™ HiPure Plasmid FP Maxiprep Kit includes Equilibration Buffer, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, RNase A, Wash Buffer, Elution Buffer, TE Buffer, HiPure Filter Columns, and a PureLink™ HiPure Precipitator Module. The PureLink™ HiPure Precipitator Module includes HiPure Precipitators, and 5 mL and 30 mL syringes. Store all components at room temperature. |
| Format | Column |
| Isolation Technology | Anion Exchange Resin |
| Sample Type | Bacterial Culture |
| Elution Volume | 15 mL |
| Final Product Type | Plasmid DNA |
| For Use With (Application) | Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| No. of Reactions | 25 Preps |
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Safety and Handling
- PureLink HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit
- Warning
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- P264-Wash face, hands and anyexposed skin thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- MIXTURE LIST-Contain: Sodium lauryl sulfate, Sodium hydroxide,acetic acid
For Research Use Only. Not for use in diagnostic procedures.
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