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Invitrogen™ pTrcHis2 TOPO™ TA Expression Kit
Allow fast, efficient cloning of PCR products into prokaryotic expression vector for high-level, regulated expression from trc promoter
Brand: Invitrogen™ K440001
Additional Details : Weight : 0.00450kg
Includes: The pTrcHis2-TOPO TA Expression Kit contains two boxes. The TOPO TA box contains 200ng of linearized, topoisomerase I-activated pTrcHis-TOPO or pTrcHis2-TOPO vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The TOP10 box contains 21 x 50μL aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and a supercoiled control plasmid.
Description
- Vector includes minicistron element for enhanced translation efficiency of eukaryotic proteins in E. coli
- Vector provided linearized and activated with topoisomerase I for 5-minute TOPO Cloning with >85% cloning efficiency
- N-terminal Xpress epitope for detection with Anti-Xpress Antibody
- N-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-HisG Antibody
- Enterokinase cleavage site for efficient removal of N-terminal fusion tags
- pTrcHis2-TOPO™ C-terminal c-myc epitope for detection with Anti-myc Antibody
- C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
Bacterial Expression/Protein Expression/Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| Trc, lacO | |
| None | |
| Ampicillin (AmpR) | |
| EK (Enterokinase) Recognition Site | |
| Cell-Based Expression | |
| TOPO-TA | |
| TOPO™ |
| IPTG | |
| TA Expression Kit | |
| The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| TOP10 | |
| E. coli | |
| His Tag (6x), c-Myc Epitope Tag | |
| pTrc |
For Research Use Only. Not for use in diagnostic procedures.