Invitrogen™ Platinum™ SuperScript™ One-Step RT-PCR System with Platinum Taq DNA Polymerase

• The SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR
• Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA
• Reverse transcription automatically follows PCR cycling without additional steps
• Using the SuperScript One-Step System The system consists of two major components: RT/Platinum Taq Mix and 2X Reaction Mix
• The RT/Platinum Taq Mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification
• SuperScript II RT is a modified version of Moloney Murine Leukemia Virus (M-MLV) RT, engineered to reduce RNase H activity and increase thermal stability
• Platinum Taq DNA polymerase is Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures
• The antibody is denatured and polymerase activity is restored during the denaturation step in PCR cycling at 94°C
• This provides an automatic “hot start” in PCR, increasing sensitivity, specificity, and yield
• The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg²⁺ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers

• Final product size: 3kb or less

One-Step RT-PCR, PCR & Real-Time PCR, RT-PCR, Reverse Transcription