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Invitrogen™ Platinum™ PCR SuperMix High Fidelity
Description
Includes
Platinum PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50μL each are provided (4 x 1.125mL). Platinum PCR SuperMix High Fidelity Components: 22U/mL complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum Taq Antibody; 66mM Tris-SO4 (pH 8.9); 19.8mM (NH4)2SO4; 2.4mM MgSO4; 220μM dNTPs; and stabilizers.
Requires
Template and primers
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.
High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.
Benefits of Platinum PCR SuperMix, High Fidelity
- Six-times higher fidelity than Taq DNA polymerase
- Amplifies fragments up to 15 kb
- Minimizes PCR optimization
- Room-temperature reaction assembly
Applications
- High-fidelity PCR
- Cloning
- Mutagenesis
Notes
- Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
- Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
Specifications
| Concentration | 1.1X |
| Content And Storage | Platinum PCR SuperMix High Fidelity (4 x 1.125 mL) Store at -20°C in a non-frost-free freezer. |
| GC-Rich PCR Performance | Low |
| Polymerase | Platinum Taq DNA Polymerase High Fidelity |
| Reaction Speed | Standard |
| Quantity | 100 rxns |
| Shipping Condition | Wet or Dry Ice |
| For Use With (Application) | Hot-start PCR, High-fidelity PCR |
| Fidelity (vs. Taq) | 6X |
| Hot Start | Built-In Hot Start |
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For Research Use Only. Not for use in diagnostic procedures.
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