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Invitrogen™ pBAD/gIII Kit

Product Code. 10655213
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Product Code. 10655213 Supplier Invitrogen™ Supplier No. V45001

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Description

Includes

T20μg each of pBAD/gIII A, B and C, 20μg of pBAD/gIII/calmodulin, 1mL 20% L-arabinose, LMG194 and TOP10 E. coli stabs.

Express recombinant proteins under the tight regulation of the araBAD promoter

  • To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space
  • Features araBAD promoter for tightly regulated expression
  • Translation initiation signals for optimized E. coliexpression
  • Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression
  • C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
  • C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
  • A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Inducible
Inducing Agent Arabinose
Promoter araBAD
Product Type Bacterial Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Protein Tag c-Myc Epitope Tag
Cloning Method Restriction Enzyme/MCS
Quantity 1 kit
Vector pBAD
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For Research Use Only. Not for use in diagnostic procedures.

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