missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Biochemical Reagents
  4. DNA Reagents
  5. PCR Cloning Kits
  6. 10625953

Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

Produktkod. 10625953
missing translation for 'changeView'
missing translation for 'orderingAttributeHoverText'
Quantity:
20 Reactions
Förpackningsstorlek
Each
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Produktkod. Quantity unitSize
10625953 20 Reactions Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 missing translation for 'options'
Denna artikel kan inte returneras. Se returpolicy
Produktkod. 10625953 missing translation for 'mfr' Invitrogen™ missing translation for 'supplierNo' K263520

Please to purchase this item. Need a web account? Register with us today!

Denna artikel kan inte returneras. Se returpolicy

Beskrivning

Innehåller

Ready-to-use TOPO vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, TOP10 or Mach1-T1R Chemically E. coli, S.O.C. Medium, and a pUC19 control plasmid.

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

  • Blunt-end PCR products clone directionally into pENTR™/SD/D-TOPO™ entry vector at greater than 90% efficiency, and include upstream Shine-Dalgarno sequence for expression in prokaryotes
  • Rapidly shuttle cloned genes between multiple vector systems
  • Reliable performance
  • For access to Gateway System, PCR amplify gene of interest and add product straight to provided topoisomerase charged pENTR™ /SD SD/SD D-TOPO vector, incubate 5 minutes and transform provided competent E. coli cells
  • Resulting attL containing clones are ready for efficient recombination with your choice of vectors
  • pENTR™ /SD SD/SD D-TOPO™ vector includes T7 gene 10 translational enhancer and ribosome binding site (RBS) for optimal expression of native protein after recombination with prokaryotic vectors
  • Also use vector for expression in other hosts systems by recombination with different destination vectors
  • Vector has M13 and T7 primer sequencing sites and attL recombination sites flanking the PCR product insertion site, allowing clones to be easily sequence verified and recombined into choice of attR containing destination vectors
  • A Kanamycin resistance gene and a pUC origin are used for selection and high-copy propagation in E. coli
  • No PCR clean up, vector preparation, or other time-intensive DNA manipulation steps
  • Four base overhang on vector pairs with four base sequence designed into forward primer used inthe PCR reaction to provide directionality to topoisomerase ligation reaction

Cloning, Gateway Cloning

TRUSTED_SUSTAINABILITY

Specifikationer

Cloning Method Directional TOPO™
Content And Storage Each Directional TOPO™ pENTR Cloning Kit contains the appropriate, ready-to-use TOPO™ vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, TOP10 or Mach1-T1R Chemically E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store Competent E. coli at -80°C. Store all other components at -20°C.
No. of Reactions 20 reactions
Product Line One Shot
Product Type TOPO Cloning Kit
Quantity 20 Reactions
Vector pENTR

For Research Use Only. Not for use in diagnostic procedures.

Produkttitel
Välj ett problem

Genom att klicka på Skicka bekräftar du att du kan bli kontaktad av Fisher Scientific angående feedbacken du har lämnat i detta formulär. Vi kommer inte att dela din information för andra ändamål. All kontaktinformation som tillhandahålls ska också underhållas i enlighet med vår Sekretesspolicy.