Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1

Description

These uniform and superparamagnetic beads are 1 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. The beads show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results. The 1µm DynabeadsMyOne have a large surface area, high capacity, efficient magnetic pull, and a slow sedimentation rate during incubation. Tailor-made for use in automated protocols where high throughput is crucial.

• Very high binding affinity of the streptavidin-biotin interaction (K_d=10^-15) is used in a vast number of applications
• Direct and fast isolation of any biotinylated molecule
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Low-charged and neutral beads, optimal for binding of proteins, peptides, and antibodies
• Their small but uniform size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule
• Low sedimentation rate, yet a high iron content, ensuring rapid magnetic separation
• Biomagnetic protocols are easily adapted to automated platforms
• High batch-to-batch reproducibility, securing consistent results

Immunoprecipitation, Protein & Peptide Purification, Protein Complex Pull-Down, Protein Sample Preparation & Protein Purification, Proteins, Expression, Isolation & Analysis, RNAi, Epigenetics & Noncoding RNA Research