Invitrogen™ Exosome-Human CD81 Isolation Reagent (from cell culture)

Obtain a highly pure exosome subset with easy protocol scalability with Exosome-Human CD81 Isolation Reagent (from cell culture). Following isolation, the specific sub-population of exosomes, ECVs can be further studied using methods such as Western blotting, qPCR, or next generation sequencing.

• Minimal hands-on time for specific exosome isolation
• Compatible with most downstream analysis
• Recent studies suggest that exosome/ECV population consists of several subsets, possibly with different functions
• Using immuno-affinity based Dynabeads magnetic separation technology, these subsets can be selectively isolated and studied
• Cell culture media must first be enriched for exosomes
• Can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation
• Following enrichment, specific exosomes are isolated by binding to surface of magnetic beads
• In addition to their high-quality sensitivity, reproducibility, and stability, Dynabeads allows you to see samples during handling due to color of magnetic beads
• When a sample tube is placed in the magnet, bead-bound exosomes are pulled to side of tube, allowing for easy separation and buffer changes
• Sample and bead volume can be easily scaled according to your sample size or downstream applications

Mix thoroughly to ensure that magnetic beads do not settle in the sample tube. A mixer which provides both tilting and rotation for optimal mixing is recommended.