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Invitrogen™ Champion™ pET100 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
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Quantity:
20 reactions
Unit Size:
Each
Description
Includes
200ng of linearized, topoisomerase I-activated Champion pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. The TOP10 box contains twenty-one 50μL aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid.
- Based on T7 expression vectors originally developed by Studier and colleagues, high-level expression is achieved because T7 RNA polymerase is more processive than native E. coliRNA polymerase and is dedicated to transcription of gene of interest
- Protein production is further enhanced in system by expression strain BL21 StarE. coli, which significantly improves stability of mRNA transcripts and increases protein expression up to ten-fold
- Simplified, efficient Directional TOPO cloning allows to quickly enter a ChampionpET Expression vector
- Features linearized, topoisomerase I-activated ChampionpET expression vectors for five-minute directional cloning
- Directional TOPO Cloning technology facilitates gene expression because proofreading enzyme is used for PCR, resulting in fewer errors in cloned genes
- Greater than 90% of clones are in correct orientation for gene expression, reducing time spent on colony screening
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Constitutive or Inducible System | Inducible |
| Inducing Agent | IPTG |
| Promoter | T7, lacO |
| Product Type | TOPO Expression Kit |
| Selection Agent (Eukaryotic) | None |
| Content And Storage | Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored. |
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
| Bacterial or Yeast Strain | BL21 Star™(DE3) |
| Cleavage | EK (Enterokinase) Recognition Site |
| Expression System | E. coli |
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For Research Use Only. Not for use in diagnostic procedures.
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