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  6. Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), eBioscience™, Invitrogen™

Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), eBioscience™, Invitrogen™

Product Code. p-7069481
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Product Code. 15267557 Supplier Invitrogen Supplier No. 14588781

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Description

Rat Monoclonal Antibody

Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha4beta7), which is a heterodimer of the the 154 kDa alpha4 subunit and the 130 kDa beta7 subunit. In mouse, the beta7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion. Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis. (Please use Functional Grade purified DATK32 (DATK-32), cat. 16-5887, in functional assays.). Applications Tested: This DATK32 (DATK-32) antibody has been tested tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Integrin alpha 4 beta 7 (LPAM-1)
Applications Flow Cytometry, Functional Assay
Classification Monoclonal
Clone DATK32 (DATK-32)
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Itga4
Gene Accession No. P26011, Q00651
Gene Alias ITGB7, Gut homing receptor beta subunit, Integrin alpha-4/beta-7, Peyer patches-specific homing receptor LPAM-1, Itga4
Gene Symbols Itga4, Itgb7
Host Species Rat
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 16401, 16421
Target Species Mouse
Content And Storage 4° C
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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