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  5. Cytiva Tissue and Cells genomicPrep Mini Spin Kit

Cytiva Tissue and Cells genomicPrep Mini Spin Kit

Product Code. 10714547 Shop All Cytiva Products
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No. of Reactions:
250 Preps
50 Preps
Unit Size:
250 reactions
50 reactions
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Product Code. No. of Reactions unitSize
10714547 250 Preps 250 reactions
10685545 50 Preps 50 reactions
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Product Code. 10714547 Supplier Cytiva Supplier No. 28904276

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Description

Includes

Spin columns prepacked with a novel silica-membrane; Lyophilized proteinase K powder; Lysis solutions, wash and elution buffers; 2mL Microcentrifuge collection tubes; Full protocol booklet with a detachable, quick reference protocol card

Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures

To minimize the incidence of DNA shearing during extraction, the illustra tissue and cells genomicPrep Mini Spin Kit includes a lysis solution with proteinase K to gently release genomic DNA from tissues or cells. The kit can be used to extract DNA from diverse tissue types such as liver, kidney, and mouse tails with yields ranging from 0.5 to 1.5μg of genomic DNA per mg of tissue. The kit consistently generates purified genomic DNA with optical density A260/280 readings of about 1.8.

  • Capable of purifying up to 1.5μg/mg of tissue from an input tissue sample range of 5 to 50mg, and up to 40μg of genomic DNA per 5 million cells depending on cell type
  • Total DNA extraction time reduced significantly to 90 minutes (including tissue disruption)
  • Produces high-quality genomic DNA with high molecular weight and less degradation
  • Validated in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests

TRUSTED_SUSTAINABILITY

Specifications

Description illustra tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures in just 90 min for tissues and 45 min for ce
For Use With (Application) Used for several downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR
Includes Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
No. of Reactions 250 Preps
Product Type illustra tissue and cells genomicPrep Mini Spin Kit
Purity 1.75
Sample Volume 5-50 mg
Test Time 90 min[Animal Tissue], 45 min[Cultured cells]

Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Respiratory sensitiser Category 1
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P405-Store locked up.
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