Cytiva PlasmidPrep Mini Spin Kit

Purify high yields of plasmid DNA in less than 10 minutes. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications. illustra plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. The typical plasmid DNA yield from a fresh 1.5mL culture of E. coli containing a high copy number plasmid is approximately 9μg. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane. The kit contains spin columns prepacked with a novel silica membrane, suspension, lysis, and neutralisation buffers containing chaotropic salts, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

• Total preparation time of less than 10 minutes represents a 50% reduction in protocol time.
• Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.
• Increased ease of use provided by minimal changes in pipetting volume from one part of the protocol to the next, fewer centrifugation steps, color-coded kit components, and lack of organic solvents.