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Invitrogen™ Mouse IL-6 ProQuantum Immunoassay Kit

Product Code. 17154293
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Quantity:
96 Tests
Unit Size:
Pack of 96
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Product Code. 17154293

Brand: Invitrogen™ A43656

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This item is not returnable. View return policy

Description

ELISA

Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

  • High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
  • Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
  • Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
  • Fast, easy workflow-2 hours from sample to answer, with no wash steps
  • No proprietary instrument to purchase-runs on any real-time PCR instrument

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; ChIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; Hybridoma growth factor; Ifnb2; IFN-beta-2; IL6; Il-6; ILg6; ILN; Interferon beta-2; Interleukin; interleukin 6; interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; Interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6
Assay Range 0.32 to 5000 pg/mL
Assay Sensitivity <0.3 pg/mL
Conjugate FAM™
Product Type ProQuantum
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) qPCR Instruments
Gene ID (Entrez) 16193
Gene Symbol Il6
Kit Contents Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Storage Requirements -20°C
Target IL-6
Test Time 30 mins
Total Assay Time 2 hrs
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