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  5. Invitrogen™ Porcine IL-12 (IL-12A) ELISA Kit

Invitrogen™ Porcine IL-12 (IL-12A) ELISA Kit

Product Code. p-7230650
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
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Product Code. 15415403

Brand: Invitrogen™ ESIL12A

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Description

ELISA

The Porcine Interleukin 12 (IL-12A) ELISA quantitates Porc IL-12A in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-12A. Principle of the method The Porcine IL-12A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q28938,Q29053
Assay Range 2.458 to 600 pg/mL
Assay Sensitivity 2.2 ng/mL
Calibration Range 2.2 ng/ml to 600 ng/ml
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Cytokines & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-13, GM-CSF, IFN-gamma, TGF beta 1, TNF alpha
Sufficient For 96 assays
Target Species Porcine
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 397053
Gene Symbol IL12A, IL12B
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Oncology
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target IL-12 p35
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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