Invitrogen™ Silencer™ siRNA Transfection II Kit

This Ambion™ kit is for the optimization of transfection protocols for specific cell types. It includes 0.4 mL of each of two transfection agents; siPORT™ Amine Transfection Agent and siPORT™ NeoFX™ Transfection Agent along with control siRNAs.

• Broad cell line compatibility-kit includes siPORT™ Amine and siPORT™ NeoFX™ transfection agents
• Faster protocol optimization-kit includes ready-to-transfect GAPDH and negative siRNA controls

The choice of transfection agent is critical for gene silencing experiments. Every transfection agent has a different delivery efficiency when used with different cell types, and the overall transfection efficiency and degree of gene silencing depend on the nature of the transfection agent/siRNA complex. Whether you are new to RNAi research or you want to expand your experiments to include different cell lines, the Silencer™ siRNA Transfection II Kit enables you to optimize your transfection protocols for specific cell types. The Silencer™ siRNA Transfection II Kit includes siPORT™ Amine Transfection Agent (a polyamine mixture) and the new siPORT™ NeoFX™ Transfection Agent (a cationic and neutral lipid mixture). These reagents support efficient, reproducible siRNA transfections in different cell types.

Furthermore, both can be used to transfect cells during subculturing-saving a full day of valuable time-without increased cytotoxicity. In addition to containing a choice of transfection agents, this kit includes a well-characterized GAPDH synthetic siRNA that can be used to optimize transfection conditions. Results can also be verified at the protein level with GAPDH-specific antibodies. A scrambled, negative control siRNA is also provided to confirm the specificity of the siRNA of interest and to demonstrate the absence of nonspecific, toxic effects.