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  5. Invitrogen™ Human Transferrin ELISA Kit

Invitrogen™ Human Transferrin ELISA Kit

Product Code. 15475133
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
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Product Code. 15475133

Brand: Invitrogen™ EHTF

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Description

ELISA

The Human Transferrin ELISA quantitates Hu Transferrin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Transferrin. Principle of the method The Human Transferrin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Transferrins (Tf) are an approximately 80kDa blood plasma glycoprotein synthesized by the liver. They are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is the primary blood iron transport protein and under normal conditions; approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.Serum transferrin may also have a further role in stimulating cell proliferation.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P02787
Assay Range 1.029 to 750 ng/mL
Assay Sensitivity 1 ng/mL
Calibration Range 1 ng/mL to 750 ng/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA pair antibody detects human Transferrin. Other species not determined.
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7018
Gene Symbol HEL-S-71 p,PRO1557,PRO2086QTL1,TF
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Cardiovascular Research
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym PRO1400, PRO1557, PRO2086QTL1, TF
Target Transferrin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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