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R&D Systems™ Human Semaphorin 3E, Carrier-Free Recombinant Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ 3239-S3B-025
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Description
The Recombinant Human Semaphorin 3E Protein is derived from NS0. The Recombinant Human Semaphorin 3E Protein has been validated for the following applications: Bioactivity.Specifications
| O15041 | |
| 9723 | |
| 25μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. | |
| (semaphorin) 3E, coll-5, KIAA0331M-SEMAH, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, Sema3E, SEMAH, SEMAHM-SemaK, semaphorin-3E | |
| SEMA3E | |
| Unconjugated |
| Lyophilized from a 0.2μm filtered solution in PBS, NaCl and Tween™ 20 with Trehalose. | |
| Observed M.W.: 85-100kDa, 66kDa and 25kDa, reducing conditions, Theoretical M.W.: 88kDa | |
| Mouse myeloma cell line,NS0-derived human Semaphorin 3E protein His24-Ser775 (Arg557Ala and Arg560Ala),with an N-terminal 10-His tag | |
| <1.0 EU per 1μg of the protein by the LAL method. | |
| Semaphorin 3E | |
| Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED50 for this effect is typically 0.04-0.24μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin D1 is coated at 5μg/mL, Recombinant Human Semaphorin 3E binds with an apparent Kd <0.4 nM. | |
| Yes |
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