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Invitrogen™ S100A8 Human ELISA Kit

Product Code. 15415123
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Quantity:
96 Tests
Unit Size:
1 set
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Product Code. 15415123

Brand: Invitrogen™ EHS100A8

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Description

Sandwich ELISA Kit

The Human S100A8 ELISA quantitates Hu S100A8 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu S100A8. Principle of the method The Human S100A8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05109
Assay Range 10.24-2500 pg/mL
Assay Sensitivity 10 pg/mL
Calibration Range 10pg/mL to 2500pg/mL
Assay Solid-phase sandwich ELISA
Conjugate Biotin
Product Type ELISA Kit
Protein Family Chemokine & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA pair antibody detects human S-100A8. Other species not determined.
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6279
Gene Symbol S100A8
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Inflammation
Sample Volume Plasma, 33 μL; Serum, 33 μL; Supernatant, 100 μL
Storage Requirements 2°C to 8°C
Synonym 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8
Target Human
Test Time 1 hr. 20 min.
Total Assay Time 4 hr. 45 min.
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