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Invitrogen™ Human PKC epsilon (aa 683-737) Control Fragment Recombinant Protein

Product Code. 30203529
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. 30203529

Brand: Invitrogen™ RP102967

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This item is not returnable. View return policy

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83725 (PA5-83725. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q02156
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 5581
Name Human PKC epsilon (aa 683-737) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias 5830406C15Rik; MGC125656; MGC125657; nPKC-epsilon; PKC e; PKC[e]; Pkce; Pkcea; PKCepsilon; PRKCE; PRKCH; protein kinase C; protein kinase C epsilon; protein kinase C epsilon type; protein kinase C, epsilon; protein kinase C, eta; R75156
Common Name PKC epsilon
Gene Symbol PRKCE
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence PPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFSYFGEDLMP
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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