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  5. Invitrogen™ Human P-Selectin (Soluble) (CD62) ELISA Kit

Invitrogen™ Human P-Selectin (Soluble) (CD62) ELISA Kit

Product Code. 15521717
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10 x 96 Tests
96 Tests
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96 tests
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15521717 96 Tests 96 tests
15591707 10 x 96 Tests Pack of 10
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Product Code. 15521717 Supplier Invitrogen™ Supplier No. BMS2194

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Description

ELISA

The Human Soluble P-Selectin (Hu sP-Sel/CD62)) ELISA quantitates Hu sP-Selectin in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sP-Selectin. Principle of the method The Human sP-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P16109
Assay Range 0.63-40.00 ng/mL
Assay Sensitivity 0.20 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Amniotic Fluid, Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias CD62; CD62 antigen-like family member P; CD62P; CD62P antigen; CD62P; CD62 homolog; FLJ45155; GMP140; GMP-140; GMRP; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; Grmp; LECAM3; leukocyte-endothelial cell adhesion molecule 3; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; PSEL; PSELECT; P-selectin; P-selectin precursor; RP1-86F14.2; selectin P; selectin P (granule membrane protein 140kDa, antigen CD62); selectin, platelet; SelpCD62; CD62 antigen-like family member P; CD62P; CD62P antigen; CD62P; CD62 homolog; FLJ45155; GMP140; GMP-140; GMRP; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; Grmp; LECAM3; leukocyte-endothelial cell adhesion molecule 3; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; PSEL; PSELECT; P-selectin; P-selectin precursor; RP1-86F14.2; selectin P; selectin P (granule membrane protein 140kDa, antigen CD62); selectin, platelet; Selp; CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL
Gene ID (Entrez) 6403
Gene Symbol SELP
Interassay CV 5.4%
Intraassay CV 7.8%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Storage Requirements 2-8°C
Target P-Selectin (CD62P)
Test Time 1 hr 20 min
Total Assay Time 2 hr 30 min
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