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  5. Invitrogen™ Human MMP9 ELISA Kit

Invitrogen™ Human MMP9 ELISA Kit

Product Code. 15571487
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
96 tests
Pack of 10
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Product Code. 15571487

Brand: Invitrogen™ BMS20162

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527.44 EUR valid until 2025-12-31
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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human MMP-9
Biotin-Conjugate anti-human MMP-9 polyclonal antibody
Streptavidin-HRP
Human MMP-9 Standard lyophilized, 30ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Human Matrix Metalloproteinase 9 (Hu MMP-9) ELISA quantitates Hu MMP-9 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusivy recognize both natural and recombinant Hu MMP-9. Principle of the method The Human MMP-9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P14780
Assay Range 0.23 to 15.0 ng/mL
Assay Sensitivity 0.05 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4318
Gene Symbol CLG4B,GELB,MANDP2,MMP-9
Interassay CV 10.2%
Intraassay CV 7.3
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Storage Requirements 2°C to 8°C
Synonym 92 kDa gelatinase, Gelatinase B, GELB
Target MMP-9
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 10 mins
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Safety and Handling

compliance-icons
Product Identifier
  • 96TEST Human MMP-9 Platinum ELISA
 Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Skin corrosion/irritation Category 1 B
 Hazard Statement
  • H314-Causes severe skin burns and eye damage.
  • H318-Causes serious eye damage.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P363-Wash contaminated clothing before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Phosphoric acid
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