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  5. Invitrogen™ Human IL-29 ELISA Kit

Invitrogen™ Human IL-29 ELISA Kit

Product Code. 15561567
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
96 tests
Pack of 10
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15561567 96 Tests 96 tests
15571567 10 x 96 Tests Pack of 10
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Product Code. 15561567 Supplier Invitrogen™ Supplier No. BMS2049

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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-29
Biotin-Conjugate anti-human IL-29 monoclonal antibody
Streptavidin-HRP
Human IL-29 Standard lyophilized, 2ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Human Interleukin-29 (Hu IL-29) ELISA quantitates Hu IL-29 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-29. Principle of the method The Human IL-29 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN-lambdas constitute a novel family of interferons that mediate the induction of anti-viral protection in a wide variety of cells. There are three members in this family, namely lambda1, lambda2, and lambda3, which are also known as IL-29, IL-28A, and IL-28B, respectively. Similar to type I IFNs, IFN-lambdas activate an intracellular signaling to promote gene expression. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex composed of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. DCs that differentiate in response to IFN-lambdas express high levels of class I and II MHC gene products, but low levels of costimulatory molecules. Moreover, these cells can specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cells with contact-dependent suppressive activity.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q8IU54
Assay Range 15.6 to 1,000 pg/mL
Assay Sensitivity 2.0 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 282618
Gene Symbol IL-29,IL29
Interassay CV 7.1%
Intraassay CV 7.6
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Synonym IFNL1, Interleukin 29
Target IL-29
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 30 mins
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