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  5. Invitrogen™ Human Interferon alpha ELISA Kit

Invitrogen™ Human Interferon alpha ELISA Kit

Product Code. p-4531687
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Quantity:
96 Tests
Unit Size:
1 set
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Product Code. 10531594

Brand: Invitrogen™ 411101

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761.52 EUR valid until 2025-12-31
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Description

ELISA

The Human Interferon alpha A,2,A/D,D,K and 4b (Hu IFNαA,IFNα2,IFNαA/D,IFNαD,IFNαK and IFNα4b ) ELISA quantitates Hu IFNα in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFNα. Principle of the method The Human IFNα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 12.5 to 1000 pg/ml
Description Human IFN alpha ELISA Kit (Serum Samples)
Conjugate HRP
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Specificity No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t
Sufficient For 96 microplate wells
Target Species Human
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3439, 3440
Gene Symbol IFNA1,IFNA2
Interassay CV <8%
Intraassay CV <8
Kit Contents Pre-coated 96 well Microplate, Detection Antibody Concentrate, Anti-IgG-HRP Concentrate, Concentrate Diluent, Standard, Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Measurement Absorbance at 450 nm
Precision Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target IFN alpha
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 15 mins
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