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Invitrogen™ Human Hemoglobin ELISA Kit

Product Code. 17727633
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Quantity:
10 x 96 Tests
Unit Size:
Pack of 10
This item is not returnable. View return policy

Product Code. 17727633

Brand: Invitrogen™ EH237RBX10

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This item is not returnable. View return policy

Description

ELISA

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P69905
Assay Range 1.5 to 400 ng/mL
Assay Sensitivity 1.5 μg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3039, 3043
Gene Symbol HBA1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Hemoglobin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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