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Gibco™ Human FGF-10 Recombinant Protein, PeproTech®

Product Code. 17810503
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Quantity:
100 μg
2 x 500 μg
25 μg
250 μg
5 μg
50 μg
500 μg
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100µg
1mg
250µg
25µg
500µg
50µg
5µg
Pack of 2
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Artikelnummer. 17810503

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Beschreibung

Recombinant Protein

100-00CC-500UG will be provided as 2 x 250 μg (100-00CC-250UG). Recombinant Human FGF-10 is a 19.3 kDa protein consisting of 170 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.
TRUSTED_SUSTAINABILITY

Spezifikation

Accession Number O15520
For Use With (Application) Control, Western Blot Control, Bioactivity
Formulation Protein with no preservative
Gene ID (Entrez) 2255
Molecular Weight (g/mol) 19.3 kDa
Name Human FGF-10
Purification Method Purified
Quantity 5 μg
Source E. coli
Storage Requirements -20°C
Regulatory Status RUO
Endotoxin Concentration <1 EU/μg
Gene Alias AEY17; BB213776; FGF; Fgf10; FGF-10; Fibroblast growth factor; Fibroblast growth factor 10; Gsfaey17; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
Common Name FGF10
Gene Symbol FGF10
Biological Activity Assay 1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg Assay 2: Determined by a cell proliferation assay using 4 MBr-5 cells. The expected ED50 for this effect is 0.5-15.0 ng/ml
Product Type Protein
Conjugate Unconjugated
Recombinant Recombinant
Sequence MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS
Content And Storage -20°C
Activity Assay 1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg. Assay 2: Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-15.0 ng/ml.
Expression System E. coli
Form Lyophilized
Purity or Quality Grade ≥ 95% by SDS-PAGE gel and HPLC analyses.
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