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Gibco™ Human EPO (Erythropoietin) Recombinant Protein

Product Code. 12313703
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Quantity:
10 μg
100 μg
Unit Size:
10µg
Each
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Product Code. 12313703

Brand: Gibco™ PHC9634

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This item is not returnable. View return policy

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, prior to opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile water to a concentration of 0.1-1 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA. Storage: Store lyophilized human EPO 2°C to 8°C, preferably desiccated. Store reconstituted human EPO at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01588
For Use With (Application) Bioactivity
Formulation Protein with no preservative
Gene ID (Entrez) 2056
Molecular Weight (g/mol) 18.4 kDa
Name Human EPO (Erythropoietin)
Purification Method Purified
Quantity 10 μg
Storage Requirements 4°C
Regulatory Status RUO
Endotoxin Concentration <0.1 ng/μg
Gene Alias EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
Common Name Epo
Gene Symbol EPO
Biological Activity ED50 ≤ 0.8 ng/mL; determined by the dose-dependent proliferation of TF-1 cells.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Content And Storage 4°C
Classification Carrier-Free
Activity ED50 ≤ 0.8 ng/mL; determined by the dose-dependent proliferation of TF-1 cells.
Bioassay Proliferation of TF1 cells
Endotoxin Level <0.1 ng/μg
Shipping Condition Wet Ice
Expression System CHO cells
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype EPO
Research Category Stem Cell Research, Neurobiology
Product Line Gibco
Purity or Quality Grade ≥95% by SDS-PAGE
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